| 质粒名称: | pMK232 (CMV-OsTIR1-PURO) |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 72834 |
| 存储实验室: | Masato Kanemaki |
|
载体骨架基本信息 |
|
| 载体名称: | pBluescript |
| 空载体大小: | 3926 bp |
| 完整质粒大小: | 9590 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | AAVS1-CMV-OsTIR1-PURO-AAVS1 |
| 物种来源: | H. sapiens (human) |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT. Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24. 10.1016/j.celrep.2016.03.001 PubMed 27052166 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pMK232 (CMV-OsTIR1-PURO) 9584 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses OsTIR1 under the control of the CMV promoter.
ACCESSION .
VERSION .
KEYWORDS pMK232 (CMV-OsTIR1-PURO)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9584)
AUTHORS Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT
TITLE Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron
Tagging with Short Homology Donors.
JOURNAL Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001.
Epub 2016 Mar 24.
PUBMED 27052166
REFERENCE 2 (bases 1 to 9584)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9584
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 295..1098
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
enhancer 1155..1458
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1459..1662
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1613..1633
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 1700..3427
/codon_start=1
/product="rice transport inhibitor response 1 protein"
/label=OsTIR1
/note="F-box protein that mediates auxin-dependent
interaction of a ubiquitin ligase with an auxin-inducible
degron (Tan et al., 2007)"
/translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
polyA_signal 3601..3722
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3638..3657)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3692..3711
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 3731..4231
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 4150..4168
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 4253..4852
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4253..4272)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4749..4769
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 4859..5083
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature 5086..5922
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
primer_bind complement(5956..5975)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5957..5975)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5982..5999)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5982..5998)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5991..6013)
/label=M13/pUC Forward
/note="In lacZ gene"
CDS 6136..6438
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 6317..6336
/label=ccdB-fwd
/note="ccdB gene, forward primer"
CDS 6787..7581
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6841..6860)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7451..7470
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
CDS complement(7831..8691)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8454..8473
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8815..9403
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9304..9323
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9557..9574
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
1081 tccaccccac agtggggcaa ttaatagtaa tcaattacgg ggtcattagt tcatagccca
1141 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
1201 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
1261 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
1321 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
1381 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
1441 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
1501 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
1561 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
1621 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
1681 atccgctagc gctgccacca tgacatactt tcctgaagag gtcgtcgaac acatttttag
1741 cttcctgcct gcacagagag atagaaacac agtgagcctg gtctgcaaag tgtggtacga
1801 gatcgaacgc ctgagccgga gaggagtgtt cgtcggcaac tgctatgctg tgagagcagg
1861 cagggtcgcc gctaggtttc caaatgtgcg cgcactgacc gtcaagggga aaccccactt
1921 cgccgacttt aacctggtgc cccctgattg gggaggatac gccggccctt ggatcgaggc
1981 agccgctcgc ggctgtcatg gactggagga actgcgcatg aagcgaatgg tggtctctga
2041 cgaaagtctg gagctgctgg ctcggagctt ccctaggttt cgcgcactgg tgctgatttc
2101 ttgcgaaggc ttcagcaccg atggactggc agccgtggcc tcccactgta agctgctgcg
2161 ggagctggac ctccaggaga atgaagtgga ggatagaggc cccagatggc tgtcttgctt
2221 cccagactca tgtaccagcc tggtgtccct gaactttgcc tgcatcaaag gcgaagtgaa
2281 tgctgggtcc ctggagcggc tggtctcaag aagccccaac ctgaggtctc tgcggctgaa
2341 ccggagcgtg agcgtggaca ctctggctaa gattctgctg agaaccccta acctggagga
2401 tctgggaacc ggcaatctga cagacgattt ccagacagaa tcctacttta aactgacttc
2461 tgccctggag aagtgtaaaa tgctgaggag tctgtcagga ttctgggatg cttcacccgt
2521 gtgcctgagc tttatctacc ctctgtgtgc acagctgaca ggcctgaacc tgagctatgc
2581 accaaccctg gacgccagtg atctgacaaa gatgatctca cgctgcgtga aactccagcg
2641 actgtgggtg ctggactgta tttccgataa ggggctccag gtggtcgcca gctcctgcaa
2701 ggacctccag gagctgagag tgttcccatc tgatttttac gtggccggat atagtgctgt
2761 cactgaggaa ggcctggtgg cagtctcact gggatgccca aagctgaaca gcctgctgta
2821 tttctgtcat cagatgacta atgctgcact ggtgaccgtc gccaagaact gccctaattt
2881 cacccgattt cggctgtgta ttctggaacc aggcaaaccc gacgtggtca catcccagcc
2941 actggatgaa gggtttggag ctatcgtgag agagtgcaag ggactccaga ggctgagcat
3001 ttccggcctg ctgacagaca aagtgttcat gtacatcggc aagtatgcta agcagctgga
3061 gatgctgagc attgcatttg ccggagactc cgataagggc atgatgcacg tgatgaacgg
3121 gtgtaagaat ctgcgaaaac tggaaatccg ggacagccct ttcggggatg ccgctctgct
3181 gggaaacttt gccagatacg agacaatgag gagcctgtgg atgtctagtt gcaatgtgac
3241 tctgaagggc tgtcaggtcc tggctagtaa aatgcctatg ctgaacgtgg aagtcattaa
3301 tgagcgggac gggtctaacg aaatggagga aaatcatggc gacctgccaa aggtggagaa
3361 actgtatgtg tatcggacca ccgcaggggc aagagatgat gctcccaact ttgtgaagat
3421 tctgtgagga tccatcggat cccgggcccg tcgacggtac cgcgggcccg ggatccaccg
3481 gggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
3541 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
3601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
3661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
3721 taagttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
3781 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
3841 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
3901 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
3961 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
4021 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
4081 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
4141 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
4201 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
4261 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
4321 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
4381 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
4441 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
4501 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
4561 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
4621 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
4681 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
4741 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
4801 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaggtaccct
4861 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
4921 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
4981 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
5041 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggttactag ggacaggatt
5101 ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat attgggtcta
5161 acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt tcctggagcc
5221 atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag aggatcctgg
5281 gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg cccggttctc
5341 agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg gctgtctggt
5401 gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag aagcagtttg
5461 gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc acctttctag
5521 tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag gccagtagcc
5581 agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa actccctttg
5641 tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc ctttctcttt
5701 ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc ctccgcccag
5761 agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt ccttggcaag
5821 cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct catgcccctg
5881 gctctcctgc cccttcccta caggggttcc tggctctgct ctaagggcga attcgcggcc
5941 gctaaattca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
6001 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
6061 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
6121 agcctatacg tacggcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
6181 tttgtggatg tacagagtga tattattgac acgccggggc gacggatggt gatccccctg
6241 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
6301 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
6361 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
6421 atgttctggg gaatataaat gtcaggcatg agattatcaa aaaggatctt cacctagatc
6481 cttttcacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
6541 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
6601 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
6661 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
6721 ccgccaagga tctgatggcg caggggatca agctctgatc aagagacagg atgaggatcg
6781 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
6841 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
6901 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
6961 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
7021 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
7081 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
7141 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
7201 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
7261 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
7321 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
7381 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
7441 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
7501 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
7561 cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg gtattttctc
7621 cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg
7681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagatt
7741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
7801 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
7861 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
7921 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
7981 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
8041 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
8101 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
8161 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
8221 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
8281 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
8341 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
8401 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
8461 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
8521 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
8581 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
8641 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
8701 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgaccaaaa tcccttaacg
8761 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
8821 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
8881 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
8941 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
9001 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
9061 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
9121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
9181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
9241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
9301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
9361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
9421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
9481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
9541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
